SOX15 Polyclonal antibody

SOX15 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16725-1-AP

Synonyms

Transcription factor SOX-15, SRY-box transcription factor 15, SOX-15, SOX 15, Protein SOX-20

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Validation Data Gallery

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  IHC staining of mouse embryo using 16725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 16725-1-AP (SOX15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 16725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 16725-1-AP (SOX15 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse embryo using 16725-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 16725-1-AP (SOX15 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse embryo using 16725-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 16725-1-AP (SOX15 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse embryo tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse embryo tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

16725-1-AP targets SOX15 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOX15 fusion protein Ag10173
• Full Name: SRY (sex determining region Y)-box 15
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC000985
• Gene Symbol: SOX15
• Gene ID (NCBI): 6665
• RRID: AB_2878305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,pig	IF	Cell Rep; Integration of single-cell transcriptome and chromatin accessibility of early gonads development among goats, pigs, macaques, and humans; Authors - Min Chen