SOX15 Polyclonal antibody

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• SOX15 Polyclonal antibody (16725-1-AP)

SOX15 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 16725-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF-P, ELISA

Synonyms

Transcription factor SOX-15, SRY-box transcription factor 15, SOX-15, SOX 15, Protein SOX-20

• 

  IHC staining of mouse embryo using 16725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 16725-1-AP (SOX15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse embryo using 16725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 16725-1-AP (SOX15 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse embryo using 16725-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 16725-1-AP (SOX15 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse embryo using 16725-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 16725-1-AP (SOX15 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: mouse embryo tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse embryo tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16725-1-AP targets SOX15 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10173

  Product name: Recombinant human SOX15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-233 aa of BC000985

  Sequence: MALPGSSQDQAWSLEPPAATAAASSSSGPQEREGAGSPAAPGTLPLEKVKRPMNAFMVWSSAQRRQMAQQNPKMHNSEISKRLGAQWKLLDEDEKRPFVEEAKRLRARHLRDYPDYKYRPRRKAKSSGAGPSRCGQGRGNLASGGPLWGPGYATTQPSRGFGYRPPSYSTAYLPGSYGSSHCKLEAPSPCSLPQSDPRLQGELLPTYTHYLPPGSPTPYNPPLAGAPMPLTHL

• Full Name: SRY (sex determining region Y)-box 15
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC000985
• Gene Symbol: SOX15
• Gene ID (NCBI): 6665
• RRID: AB_2878305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60248
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The SOX15 Polyclonal antibody (Cat# 16725-1-AP) has 1 total citation. It was cited in Cell Reports (2022), in a study on single-cell transcriptome and chromatin accessibility during early gonad development across multiple species, including humans and pigs. The research field is reproductive/developmental biology, with the antibody applied in immunofluorescence (IF) for human and pig samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,pig	IF	Cell Rep; Integration of single-cell transcriptome and chromatin accessibility of early gonads development among goats, pigs, macaques, and humans; Authors - Min Chen