SOX6 Polyclonal antibody

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  WB analysis using 14010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14010-1-AP (SOX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14010-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14010-1-AP (SOX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14010-1-AP (SOX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SOX6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14010-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

HSSOX6, SOXD, TOLCAS, Transcription factor SOX-6

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Tested Applications

• Positive WB detected in: U-251 cells, Jurkat cells, C6 cells
• Positive IHC detected in: mouse testis tissue, mouse skin tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

14010-1-AP targets SOX6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOX6 fusion protein Ag5111
• Full Name: SRY (sex determining region Y)-box 6
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC047064
• Gene Symbol: SOX6
• Gene ID (NCBI): 55553
• RRID: AB_2196095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35712
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Sex-determining Region Y (SRY)-related high-mobilitygroup box 6 (SOX6) is a transcription factor that was discovered in 1990s while searching for Sry-related genes. SOX6 belongs to the subfamily D of SOX protein and can recognize the conserved sequence of "(A/T)(A/T)CAA(A/ T)G" within the transcriptional regulation region of the target gene with its HMG domain. Sox6 contains DNA binding domain and binds to the minor groove of DNA. The ability of SOX6 to interact with different proteins at different places and times, makes it an intricate protein with roles in multiple processes. SOX6 has been implicated in diabetic nephropathy, adipogenesis, cardiomyopathy, inflammatory bowel disease, and cancer. (PMID: 33230925)

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	WB	Nat Commun; VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.; Authors - Yuki Miyamoto
mouse	WB	Int J Mol Med; miRNA‑342 suppresses renal interstitial fibrosis in diabetic nephropathy by targeting SOX6.; Authors - Zhen-Huan Jiang
human	WB	Nephrology (Carlton); CircHOMER1 aggravates oxidative stress, inflammation and ECM deposition in high glucose-induced human mesangial cells; Authors - Shi Shu
	IF	bioRxiv; Eed controls craniofacial osteoblast differentiation and mesenchymal proliferation from the neural crest; Authors - Tim Casey-Clyde
human	WB	Matrix Biol; SERPINA3 is a marker of cartilage differentiation and is essential for the expression of extracellular matrix genes during early chondrogenesis; Authors - Matthew J Barter