SOX7 Polyclonal antibody

SOX7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 23925-1-AP

Synonyms

Transcription factor SOX-7, Transcription factor SOX 7, SOX 7, endoderm lineage markers

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Validation Data Gallery

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  WB analysis using 23925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 23925-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 23925-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of PC-3 using 23925-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 23925-1-AP (SOX7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 23925-1-AP (SOX7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23925-1-AP (SOX7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23925-1-AP (SOX7 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of U2OS using 23925-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SOX7 antibody (23925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse heart tissue, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

23925-1-AP targets SOX7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOX7 fusion protein Ag10323
• Full Name: SRY (sex determining region Y)-box 7
• Calculated Molecular Weight: 388 aa, 42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC071947
• Gene Symbol: SOX7
• Gene ID (NCBI): 83595
• RRID: AB_2879363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOX7, also named as Transcription factor SOX-7, is a 388 amino acid protein, which is widely expressed in adult and fetal tissues and tends to be down-regulated in prostate adenocarcinomas and colorectal tumors due to promoter hypermethylation. SOX7 binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors. SOX7 may be required for the survival of both hematopoietic and endothelial precursors during specification.

Publications

Species	Application	Title
human	WB	Oncogene; SOX7 regulates MAPK/ERK-BIM mediated apoptosis in cancer cells.; Authors - Qiao-Yang Sun
human	WB,IP	Mol Genet Genomics; Predisposition to atrioventricular septal defects may be caused by SOX7 variants that impair interaction with GATA4.; Authors - Baolei Li
human	WB	Cell Death Dis; The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.; Authors - Nanchao Hong
human,mouse	WB,IF	FASEB J; System analysis based on weighted gene co-expression analysis identifies SOX7 as a novel regulator of hepatic stellate cell activation and liver fibrosis; Authors - Yuwei Liu