SOX9 Polyclonal antibody

• 

  WB analysis using 55152-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55152-1-AP (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SOX9 Polyclonal Antibody for WB, ELISA

Cat No. 55152-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1

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Tested Applications

• Positive WB detected in: HepG2 cells, TT cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

55152-1-AP targets SOX9 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SRY (sex determining region Y)-box 9
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56 kDa, 68-75 kDa
• GenBank Accession Number: NM_000346
• Gene Symbol: SOX9
• Gene ID (NCBI): 6662
• RRID: AB_3670240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48436
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor. SOX9 can be ubiquitinated or SUMOylated to higher molecular weight (PMID: 24155239; 16307912; 16554309; 32070068)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB,IHC	Stem Cell Res Ther; Tensile stress promotes the chondrogenic ability of condylar cartilage stem/progenitor cells in the temporomandibular joint via the Piezo1-Ca2+-Prkca pathway; Authors - Wuyi Gong
human,mouse	WB	J Adv Res; Genomic landscape of paired primary and peritoneal metastatic lesions in gastric cancer highlights evolutionary dynamics and mutational drivers; Authors - Song Li
mouse	WB	Nat Commun; Astrocytic pleiotrophin deficiency in the prefrontal cortex contributes to stress-induced depressive-like responses in male mice; Authors - Dongmei Chi
rat	WB	J Craniofac Surg; FSTL1 Can Be a Promising Target in TMJ Osteoarthritis via Regulating Chondrocyte Mitophagy and Apoptosis; Authors - Fangjie Li
human	IF	Tissue Cell; Expression pattern of RNA demethylase ALKBH5 in fetal and adult human testis; Authors - Fang Fang
mouse	IHC	Metabolism; The chemokine CCL20 promotes hepatocyte cholesterol deposition during metabolic dysfunction-associated steatohepatitis by regulating OLR1 expression; Authors - Min Yin