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Anti-Human SOX9 Rabbit Recombinant Antibody, PBS Only (98586-1-PBS)

Anti-Human SOX9 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human SOX9 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98586-1-PBS-1MG

SOX9 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98586-1-PBS

Clone No.251471F12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CMD1, CMPD1, SRA1, Transcription factor SOX 9, Transcription factor SOX-9

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllFC (Intra)

FC experiment of SW480 using 98586-1-RR (same clone as 98586-1-PBS)
1x10^6 SW480 cells were intracellularly stained with 0.25 ug Anti-Human SOX9 Rabbit RecAb (98586-1-RR, Clone: 251471F12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98586-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 SW480 cells were intracellularly stained with 0.25 ug Anti-Human SOX9 Rabbit RecAb (98586-1-RR, Clone: 251471F12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98586-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 98586-1-RR (same clone as 98586-1-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SOX9 Rabbit RecAb (98586-1-RR, Clone: 251471F12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98586-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SOX9 Rabbit RecAb (98586-1-RR, Clone: 251471F12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98586-1-PBS in a different storage buffer formulation.
FC experiment of PC-3 using 98586-1-RR (same clone as 98586-1-PBS)
1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human SOX9 Rabbit RecAb (98586-1-RR, Clone: 251471F12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98586-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 PC-3 cells were intracellularly stained with 0.25 ug Anti-Human SOX9 Rabbit RecAb (98586-1-RR, Clone: 251471F12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98586-1-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98586-1-PBS targets SOX9 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	SRY (sex determining region Y)-box 9
Calculated Molecular Weight	56 kDa
GenBank Accession Number	NM_000346
Gene Symbol	SOX9
Gene ID (NCBI)	6662
RRID	AB_3746198
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P48436
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.