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SP1 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66508-1-Ig |CloneNo.: 4G3H3

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

SP1 Antibody WB Jurkat cells 66508-1-Ig

Various cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

SP1 Antibody IHC human lung cancer tissue 66508-1-Ig

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig( SP1 antibody) at dilution of 1:350 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
Human
Positive WB detected in:
Jurkat cells, NIH/3T3 cells
Positive IHC detected in:
human lung cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:175-1:700
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SP1 fusion protein Ag16720
Full name:
Sp1 transcription factor
Calculated molecular weight:
785aa,81 kDa
Observed molecular weight:
95-105, 60-65 kDa
GenBank accession number:
BC062539
Gene ID (NCBI):
6667
Gene symbol
SP1
Synonyms
SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1

Background

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].


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