SP1 Monoclonal antibody

SP1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

4G3H3

Cat no : 66508-1-Ig

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1



Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:175-1:700
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

66508-1-Ig targets SP1 in WB, IHC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SP1 fusion protein Ag16720
Full Name Sp1 transcription factor
Calculated molecular weight 785 aa, 81 kDa
Observed molecular weight 95-105 kDa, 60-65 kDa
GenBank accession numberBC062539
Gene symbol SP1
Gene ID (NCBI) 6667
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].

Protocols

Product Specific Protocols
WB protocol for SP1 antibody 66508-1-IgDownload protocol
IHC protocol for SP1 antibody 66508-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Evid Based Complement Alternat Med

Guifu Dihuang Pills Ameliorated Mucus Hypersecretion by Suppressing Muc5ac Expression and Inactivating the ERK-SP1 Pathway in Lipopolysaccharide/Cigarette Smoke-Induced Mice

Authors - Huanhuan Zhang