SP1 Monoclonal antibody
SP1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4G3H3
Cat no : 66508-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, NIH/3T3 cells, HEK-293 cells, HeLa cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:175-1:700 |
Sample-dependent, check data in validation data gallery |
Product Information
66508-1-Ig targets SP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | SP1 fusion protein Ag16720 |
Full Name | Sp1 transcription factor |
Calculated molecular weight | 785 aa, 81 kDa |
Observed molecular weight | 95-105 kDa, 60-65 kDa |
GenBank accession number | BC062539 |
Gene symbol | SP1 |
Gene ID (NCBI) | 6667 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].
Protocols
Product Specific Protocols | |
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WB protocol for SP1 antibody 66508-1-Ig | Download protocol |
IHC protocol for SP1 antibody 66508-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |