SP2 Polyclonal antibody

SP2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 25000-1-AP

Synonyms

KIAA0048, SP2, Sp2 transcription factor, Transcription factor Sp2

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 25000-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25000-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 25000-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25000-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, K-562 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 25000-1-AP is SP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SP2 fusion protein Ag8060
• Full Name: Sp2 transcription factor
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC016680
• Gene Symbol: SP2
• Gene ID (NCBI): 6668
• RRID: AB_2879839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SP2 also named as Sp2 transcription factor or KIAA0048 is a 613 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the sp1 C2H2-type zinc finger protein family. SP2 binds to box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites in nucleus . SP2 may regulate expression of the T-cell antigen receptor (TCR) gene.

Publications

Species	Application	Title
human	IHC	Commun Biol; ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer; Authors - Rebecca Garnham

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Anh (Verified Customer) (07-15-2021)

have some unspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: adult mouse cardiomyocytes

SP2 Antibody Western Blot validation (1:500 dilution) in adult mouse cardiomyocytes (Cat no:25000-1-AP)