SPAG4-Specific Recombinant monoclonal antibody

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  IHC staining of mouse testis using 84842-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84842-1-RR (SPAG4-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 84842-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84842-1-RR (SPAG4-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPAG4-Specific Recombinant Antibody for IHC, ELISA

Cat No. 84842-1-RR

Clone No.241920E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

SPAG4, SUN domain-containing protein 4, Sperm-associated antigen 4 protein, sperm associated antigen 4, Outer dense fiber-associated protein SPAG4

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84842-1-RR targets SPAG4-Specific in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: sperm associated antigen 4
• Calculated Molecular Weight: 48 kDa
• GenBank Accession Number: NM_003116
• Gene Symbol: SPAG4
• Gene ID (NCBI): 6676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NPE6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPAG4 may assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.