SPAG6 Polyclonal antibody

SPAG6 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12462-1-AP

Synonyms

pf16, Protein PF16 homolog, Repro SA 1, SPAG6, sperm associated antigen 6, Sperm flagellar protein

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Validation Data Gallery

• 

  WB analysis of mouse testis using 12462-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12462-1-AP (SPAG6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12462-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12462-1-AP (SPAG6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12462-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12462-1-AP (SPAG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12462-1-AP

  IP result of anti-SPAG6 (IP:12462-1-AP, 3ug; Detection:12462-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, Jurkat cells, PC-3 cells
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 3 publications below

Product Information

12462-1-AP targets SPAG6 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPAG6 fusion protein Ag3130
• Full Name: sperm associated antigen 6
• Calculated Molecular Weight: 458 aa, 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC030585
• Gene Symbol: SPAG6
• Gene ID (NCBI): 9576
• RRID: AB_2194798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sperm-associated antigen 6 (SPAG6) is an axonemal protein ubiquitously expressed in tissues containing ciliated cells. SPAG6 is essential for sperm motility and fertility. As a novel cancer-testis antigen, SPAG6 is overexpressed in myeloid malignancies. Recently, SPAG6 has been found to negatively regulate neuronal migration during mouse brain development.

Publications

Species	Application	Title
human	IF	Front Endocrinol (Lausanne); Novel Loss-of-Function Mutations in DNAH1 Displayed Different Phenotypic Spectrum in Humans and Mice.; Authors - Ranjha Khan
mouse	WB, IF	J Mol Neurosci; Spag6 Negatively Regulates Neuronal Migration During Mouse Brain Development.; Authors - Runchuan Yan
human	WB	Ecotoxicol Environ Saf; Microcystin leucine arginine induces human sperm damage: Involvement of the Ca2+/CaMKKβ/AMPK pathway; Authors - Xing Guo
mouse	WB	EBioMedicine; CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men; Authors - Hui-Juan Jin
human	IF	J Assist Reprod Genet; Identification of novel biallelic LRRC6 variants in male Chinese patients with primary ciliary dyskinesia and infertility; Authors - Yunhao Li
human	WB	Clin Genet; A homozygous ARMC3 splicing variant causes asthenozoospermia and flagellar disorganization in a consanguineous family; Authors - Fazal Rahim