SPARCL1 Polyclonal antibody

SPARCL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Publications

9

Cat no : 13517-1-AP

Synonyms

Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1

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Validation Data Gallery

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  WB analysis of human heart using 13517-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13517-1-AP (SPARCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 13517-1-AP

  IP result of anti-SPARCL1 (IP:13517-1-AP, 4ug; Detection:13517-1-AP 1:300) with Raji cells lysate 1440ug.

• 

  IHC staining of human tonsillitis using 13517-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13517-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13517-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 13517-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human heart tissue
• Positive IP detected in: Raji cells
• Positive IHC detected in: human tonsillitis tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13517-1-AP targets SPARCL1 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPARCL1 fusion protein Ag4448
• Full Name: SPARC-like 1 (hevin)
• Calculated Molecular Weight: 664 aa, 75 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC033721
• Gene Symbol: SPARCL1
• Gene ID (NCBI): 8404
• RRID: AB_10598475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.

Publications

Species	Application	Title
mouse	WB	CNS Neurosci Ther; Endothelial genetic deletion of CD147 induces changes in the dual function of the blood-brain barrier and is implicated in Alzheimer's disease.; Authors - Hao Wang
		J Neurochem; Defective Synthesis and Release of Astrocytic Thrombospondin-1 Mediates the Neuronal TDP-43 Proteinopathy Resulting in Defects in Neuronal Integrity Associated with Chronic Traumatic Encephalopathy: In Vitro Studies.; Authors - A R Jayakumar
human	IHC	Cancer Biomark; Integrated analysis reveals down-regulation of SPARCL1 is correlated with cervical cancer development and progression; Authors - Dong-Ming Wu
human	WB	Oral Dis; FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization; Authors - Xiangrui Ma
mouse	IF	PLoS One; Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility.; Authors - Ling Yu
mouse	WB	J Chem Neuroanat; Olfactory ensheathing cells facilitate neurite sprouting and outgrowth by secreting high levels of hevin.; Authors - Lite Ge