SPATA18 Polyclonal antibody, PBS Only

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• SPATA18 Polyclonal antibody, PBS Only (31808-1-PBS)

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  WB analysis using 31808-1-AP (same clone as 31808-1-PBS)

  Mouse testis tissue lysate was subjected to SDS PAGE followed by western blot with 31808-1-AP (SPATA18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31808-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 31808-1-AP (same clone as 31808-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 31808-1-AP (SPATA18 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31808-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 31808-1-AP (same clone as 31808-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 31808-1-AP (SPATA18 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31808-1-PBS in a different storage buffer formulation.

SPATA18 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 31808-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

MIEAP, Mitochondria-eating protein, Spermatogenesis-associated protein 18

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Product Information

31808-1-PBS targets SPATA18 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36227

  Product name: Recombinant human SPATA18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 102-332 aa of BC025396

  Sequence: LQDTFDRERHKDPSPRDRDMQQLDSNLNSTRSQCNQVQDDLVETEKNLEESKNRSAISLLAAEEEINQLKKQLKSLQAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNADQQDTEAMSDYKKQLRNLKEEIAVLSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSASPSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQARLDAQCLLRRCIDKAETVQ

• Full Name: spermatogenesis associated 18 homolog (rat)
• Calculated Molecular Weight: 538 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC025396
• Gene Symbol: SPATA18
• Gene ID (NCBI): 132671
• RRID: AB_3742481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8TC71
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The modulation of mitophagy after DNA damage is independent of the type of DNA damage stimuli used and the protein spermatogenesis-associated protein 18 (SPATA18) is an important player in this process (PMID: 32453416; 21264221). SPATA18, also known as MIEAP, is a p53 transcriptional target and was previously reported to contribute to mitochondrial quality control by inducing intramitochondrial lysosome-like organelles after mitochondrial damage (PMID: 21264221).