SPATA22 Polyclonal antibody, PBS Only

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• SPATA22 Polyclonal antibody, PBS Only (16989-1-PBS)

• 

  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16989-1-AP (same clone as 16989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 16989-1-AP (same clone as 16989-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  IP result of anti-SPATA22 (IP:16989-1-AP, 3ug; Detection:16989-1-AP 1:500) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SPATA22 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16989-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

NYD SP20, SPATA 22, Spermatogenesis-associated protein 22, Testis development protein NYD-SP20

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Product Information

16989-1-PBS targets SPATA22 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10591

  Product name: Recombinant human SPATA22 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-363 aa of BC029483

  Sequence: MKRSLNENSARSTAGCLPVPLFNQKKRNRQPLTSNPLKDDSGISTPSDNYDFPPLPTDWAWEAVNPELAPVMKTVDTGQIPHSVSRPLRSQDSVFNSIQSNTGRSQGGWSYRDGNKNTSLKTWNKNDFKPQCKRTNLVANDGKNSCPMSSGAQQQKQLRTPEPPNLSRNKETELLRQTHSSKISGCTMRGLDKNSALQTLKPNFQQNQYKKQMLDDIPEDNTLKETSLYQLQFKEKASSLRIISAVIESMKYWREHAQKTVLLFEVLAVLDSAVTPGPYYSKTFLMRDGKNTLPCVFYEIDRELPRLIRGRVHRCVGNYDQKKNIFQCVSVRPASVSEQKTFQAFVKIADVEMQYYINVMNET

• Full Name: spermatogenesis associated 22
• Calculated Molecular Weight: 363 aa, 41 kDa
• Observed Molecular Weight: 44, 41 kDa
• GenBank Accession Number: BC029483
• Gene Symbol: SPATA22
• Gene ID (NCBI): 84690
• RRID: AB_2286640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NHS9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks.