SPATA6 Polyclonal antibody

SPATA6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11849-1-AP

Synonyms

HASH, SPATA6, spermatogenesis associated 6, SRF 1, SRF1

---

Validation Data Gallery

• 

  WB analysis of mouse testis using 11849-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11849-1-AP (SPATA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 11849-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11849-1-AP (SPATA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11849-1-AP

  IP result of anti-SPATA6 (IP:11849-1-AP, 4ug; Detection:11849-1-AP 1:800) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human testis using 11849-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11849-1-AP (SPATA6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11849-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11849-1-AP (SPATA6 Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse testis tissue, mouse ovary tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

11849-1-AP targets SPATA6 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPATA6 fusion protein Ag2413
• Full Name: spermatogenesis associated 6
• Calculated Molecular Weight: 472 aa, 54 kDa
• Observed Molecular Weight: 54-56 kDa
• GenBank Accession Number: BC020660
• Gene Symbol: SPATA6
• Gene ID (NCBI): 54558
• RRID: AB_11232594
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Toxicol Appl Pharmacol; Low dose lead exposure at the onset of puberty disrupts spermatogenesis-related gene expression and causes abnormal spermatogenesis in mouse.; Authors - Jie Xie
	IHC, IF, WB	Theriogenology; Effects of SPATA6 on proliferation, apoptosis and steroidogenesis of Hu sheep Leydig cells in vitro.; Authors - Xiaodan Li
human	WB,IF,CoIP	Clin Genet; Identification of nonfunctional SPATA20 causing acephalic spermatozoa syndrome in humans; Authors - Xiang Wang
mouse	WB	Nature; A male germ-cell-specific ribosome controls male fertility; Authors - Huiling Li