SPC25 Recombinant monoclonal antibody

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• SPC25 Recombinant monoclonal antibody (87323-1-RR)

• 

  WB analysis using 87323-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87323-1-RR (SPC25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  Affinity of 87323-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87323-1-RR against Human SPC25 were performed. The affinity constant is 34.6 pM.

SPC25 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87323-1-RR

Clone No.252476G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

AD024, hSpc25, Kinetochore protein Spc25, SPBC25

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, SKOV-3 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87323-1-RR targets SPC25 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag23985

  Product name: Recombinant human SPC25 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-224 aa of BC022255

  Sequence: MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN

• Full Name: SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC022255
• Gene Symbol: SPC25
• Gene ID (NCBI): 57405
• RRID: AB_3745470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HBM1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spindle Pole Body Component 25 (SPC25), a core subunit of the NDC80 kinetochore complex, is essential for accurate chromosome alignment and segregation during mitosis . By linking microtubules to kinetochores, SPC25 ensures proper attachment and tension generation, critical for the maintenance of genomic stability . SPC25 is largely found in the cytoplasm and nucleus. The NDC80 complex, which is involved in kinetochore-microtubule interactions and spindle assembly checkpoint activity, is composed of four subunits: SPC24, SPC25, NDC80, and NUF2. (PMID: 40587622 ,PMID: 40400636)