SPCS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SPCS2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 14872-1-AP

Synonyms

SPC25, SPase 25 kDa subunit, KIAA0102

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Validation Data Gallery

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  WB analysis using 14872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14872-1-AP (SPCS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 14872-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14872-1-AP (SPCS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 14872-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14872-1-AP (SPCS2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14872-1-AP

  IP result of anti-SPCS2 (IP:14872-1-AP, 4ug; Detection:14872-1-AP 1:2000) with HeLa cells lysate 1200 ug.

• 

  IP experiment of mouse colon using 14872-1-AP

  IP result of anti-SPCS2 (IP:14872-1-AP, 4ug; Detection:14872-1-AP 1:2000) with mouse colon tissue lysate 800 ug.

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  IHC staining of human skin cancer using 14872-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14872-1-AP (SPCS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14872-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14872-1-AP (SPCS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human colon tissue, mouse stomach tissue, rat colon tissue, mouse spleen tissue, mouse colon tissue, Jurkat cells
• Positive IP detected in: HeLa cells, mouse colon tissue
• Positive IHC detected in: human skin cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

14872-1-AP targets SPCS2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPCS2 fusion protein Ag6640
• Full Name: signal peptidase complex subunit 2 homolog (S. cerevisiae)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC064957
• Gene Symbol: SPCS2
• Gene ID (NCBI): 9789
• RRID: AB_2255321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal peptidase complex subunit 2 (SPCS2) is multi-pass membrane protein as a component of the microsomal signal peptidase complex, which function to remove signal peptides from nascent proteins before translocation into the endoplasmic reticulum lumen. This protein was evidenced as a 25 kDa protein with function not well known.

Publications

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang ; KO Validated
human	WB	EMBO J; Intramembrane client recognition potentiates the chaperone functions of calnexin; Authors - Nicolas Bloemeke

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Matthias (Verified Customer) (10-09-2019)

Strong band at ca. 20 kDa; fainter, most likely non specific, at ca. 25 kDa; some background, but overall decent antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

SPCS2 Antibody Western Blot, validation (1:1000 dilution) in 293T (Cat no:14872-1-AP)