SPDYA Polyclonal antibody

SPDYA Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17836-1-AP

Synonyms

hSpy/Ringo A, RINGO A, Ringo3, SPDY1, SPDYA, Speedy 1, Speedy protein A, SPY1

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Validation Data Gallery

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  WB analysis of Neuro-2a using 17836-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17836-1-AP (SPDYA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17836-1-AP

  IP result of anti-SPDYA (IP:17836-1-AP, 3ug; Detection:17836-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: Neuro-2a cells
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17836-1-AP is SPDYA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPDYA fusion protein Ag12041
• Full Name: speedy homolog A (Xenopus laevis)
• Calculated Molecular Weight: 313 aa, 36 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC093005
• Gene Symbol: SPDYA
• Gene ID (NCBI): 245711
• RRID: AB_2878448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPDYA, also named as SPDY1, SPY1 and RINGO A, is a cell cycle regulator which regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP. It has two isoforms with MW 33 kDa and 36 kDa.