Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, MCF-7 cells，MDA-MB-231 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10670-1-AP targets SPHK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1051 Product name: Recombinant human SPHK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 85-384 aa of BC008040 Sequence: HEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL Predict reactive species |
| Full Name | sphingosine kinase 1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC008040 |
| Gene Symbol | SPHK1 |
| Gene ID (NCBI) | 8877 |
| RRID | AB_2195809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SPHK1 antibody 10670-1-AP | Download protocol |
| IHC protocol for SPHK1 antibody 10670-1-AP | Download protocol |
| IP protocol for SPHK1 antibody 10670-1-AP | Download protocol |
| WB protocol for SPHK1 antibody 10670-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SPHK1 Polyclonal antibody (Cat# 10670-1-AP) has 92 citations. These appear across journals including Nature Aging, Cancer Communications, Autophagy, Cell Death & Disease, Oncogene, FASEB Journal, Journal of Biological Chemistry, Redox Biology, and Frontiers in Immunology, among others. Research fields span oncology (colorectal, ovarian, breast, and liver cancers), fibrosis, neurodegeneration, cardiovascular disease, inflammation, and sphingolipid metabolism. Applications are predominantly Western blot, with immunohistochemistry, immunofluorescence, and co-immunoprecipitation also represented.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis. | ||
Nat Aging Modulating mTOR-dependent astrocyte substate transitions to alleviate neurodegeneration | ||
Autophagy Mitophagy-mediated S1P facilitates muscle adaptive responses to endurance exercise through SPHK1-S1PR1/S1PR2 in slow-twitch myofibers
| ||
Redox Biol YTHDF1 mitigates acute kidney injury via safeguarding m6A-methylated mRNAs in stress granules of renal tubules | ||
Nat Cardiovasc Res Sphingosine kinase 1 is integral for elastin deficiency-induced arterial hypermuscularization. | ||
Pharmacol Res Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application on mouse heart tissue. The reviewer reported a strong and specific band at the expected molecular weight, indicating reliable performance for their experiment. Overall, the feedback is positive, confirming the antibody's specificity and signal strength in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wei (Verified Customer) (03-12-2020) | Strong and specific band at correct size for western blot.
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