SPINT1/HAI-1 Recombinant antibody

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  WB analysis using 86138-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86138-1-RR (SPINT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 86138-1-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 86138-1-RR (SPINT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86138-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86138-1-RR (SPINT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SPINT1/HAI-1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86138-1-RR

Clone No.250560F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

HAI 1, HAI-1, SPINT1, HAI, HAI1

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Tested Applications

• Positive WB detected in: T-47D cells, MCF-7 cells, mouse small intestine tissue, mouse colon tissue, Daudi cells, human placenta

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86138-1-RR targets SPINT1/HAI-1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SPINT1/HAI-1 fusion protein Eg3131
• Full Name: serine peptidase inhibitor, Kunitz type 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58-70 kDa
• GenBank Accession Number: NM_181642.2
• Gene Symbol: HAI-1
• Gene ID (NCBI): 6692
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43278-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662).