SPIRE1 Polyclonal antibody

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  WB analysis using 11295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11295-1-AP (SPIRE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 11295-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11295-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

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  IHC staining of human lung cancer using 11295-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

SPIRE1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11295-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA1135, Protein spire homolog 1, Spir 1, SPIR1, Spir-1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, SH-SY5Y cells
• Positive IHC detected in: human lung cancer tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11295-1-AP targets SPIRE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPIRE1 fusion protein Ag1798
• Full Name: spire homolog 1 (Drosophila)
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 50-60 kDa, 95 kDa
• GenBank Accession Number: BC016825
• Gene Symbol: SPIRE1
• Gene ID (NCBI): 56907
• RRID: AB_3085370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08AE8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPIRE1, Protein spire homolog 1, belongs to the family of Wiskott-Aldrich homology region-2 (WH2) proteins (PMID: 11747823). SPIRE1 acts as an actin nucleation factor, associated with the slow-growing pointed end of the new filament (PMID: 11747823, PMID: 21620703). SPIRE1 and FMN2 promote assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PMID: 26287480).