SPIRE2 Polyclonal antibody

SPIRE2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17757-1-AP

Synonyms

KIAA1832, Protein spire homolog 2, Spir 2, SPIR2, spire homolog 2 (Drosophila), SPIRE2

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Validation Data Gallery

• 

  WB analysis of mouse cerebellum using 17757-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17757-1-AP (SPIRE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 17757-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17757-1-AP (SPIRE2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

17757-1-AP targets SPIRE2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPIRE2 fusion protein Ag12117
• Full Name: spire homolog 2 (Drosophila)
• Calculated Molecular Weight: 666 aa, 74 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC111030
• Gene Symbol: SPIRE2
• Gene ID (NCBI): 84501
• RRID: AB_2239753
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IP,IHC	J Proteome Res; Comparative proteomic profiling of dystroglycan-associated proteins in wild type, mdx, and Galgt2 transgenic mouse skeletal muscle.; Authors - Yoon Jung Hae JH
mouse	WB,IF	Neuroscience; Decreased Spire2 expression is involved in epilepsy; Authors - Lixia Hao