SPOCK2 Polyclonal antibody

SPOCK2 Polyclonal Antibody for IHC, ELISA

Cat No. 11725-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

KIAA0275, SPOCK2, testican 2, TICN2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllIHC

IHC staining of human testis using 11725-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11725-1-AP (SPOCK2 Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 11725-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11725-1-AP (SPOCK2 Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 11725-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 11725-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 11725-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 11725-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 11725-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 11725-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in

human testis tissue, human kidney tissue, human pancreas cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11725-1-AP targets SPOCK2 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2313 Product name: Recombinant human SPOCK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-380 aa of BC023558 Sequence: ALAEGDAKGLKEGETPGNFMEDEQWLSSISQYSGKIKHWNRFRDEVEDDYIKSWEDNQQGDEALDTTKDPCQKVKCSRHKVCIAQGYQRAMCISRKKLEHRIKQPTVKLHGNKDSICKPCHMAQLASVCGSDGHTYSSVCKLEQQACLSSKQLAVRCEGPCPCPTEQAATSTADGKPETCTGQDLADLGDRLRDWFQLLHENSKQNGSASSVAGPASGLDKSLGASCKDSIGWMFSKLDTSADLFLDQTELAAINLDKYEVCIRPFFNSCDTYKDGRVSTAEWCFCFWREKPPCLAELERIQIQEAAKKKPGIFIPSCDEDGYYRKMQCDQSSGDCWCVDQLGLELTGTRTHGSPDCDDIV Predict reactive species
Full Name	sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2
Calculated Molecular Weight	424 aa, 47 kDa
GenBank Accession Number	BC023558
Gene Symbol	SPOCK2
Gene ID (NCBI)	9806
RRID	AB_2255261
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92563
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SPOCK2 antibody 11725-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The SPOCK2 Polyclonal antibody (Cat# 11725-1-AP) has 2 total citations. These appear in Diagnostics (Basel) and bioRxiv. The cited research spans two fields: oncology/immunology, specifically T-cell exhaustion signatures in pancreatic cancer, and neuroscience, focusing on transcriptional heterogeneity in human oligodendrocyte precursor cells. Both studies utilized the antibody for IHC applications in human samples.

Species	Application	Title
human	IHC	Diagnostics (Basel) Identification and Validation of T-Cell Exhaustion Signature for Predicting Prognosis and Immune Response in Pancreatic Cancer by Integrated Analysis of Single-Cell and Bulk RNA Sequencing Data Authors - Yaowu Zhu View Article
human	IHC	bioRxiv Transcriptional Heterogeneity Reveals a Synaptic Gene Program in Developing and Adult Human Oligodendrocyte Precursor Cells. Authors - Aliya R. Grinberg View Article