SPON2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SPON2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20513-1-AP

Synonyms

DIL 1, DIL1, M spondin, Mindin, SPON2, Spondin 2

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Validation Data Gallery

• 

  WB analysis of LNCaP using 20513-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 20513-1-AP (SPON2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20513-1-AP (SPON2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20513-1-AP (SPON2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 20513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20513-1-AP (SPON2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: LNCaP cells
• Positive IHC detected in: human prostate cancer tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20513-1-AP is SPON2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPON2 fusion protein Ag14337
• Full Name: spondin 2, extracellular matrix protein
• Calculated Molecular Weight: 331 aa, 36 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC002707
• Gene Symbol: SPON2
• Gene ID (NCBI): 10417
• RRID: AB_2878696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).

Publications

Species	Application	Title
human	WB	J Cell Mol Med; Spondin 2 promotes the proliferation, migration and invasion of gastric cancer cells.; Authors - Haoming Lu ; KD Validated
human,mouse	WB,IF	Front Cell Dev Biol; Transcriptional Activation of Matricellular Protein Spondin2 (SPON2) by BRG1 in Vascular Endothelial Cells Promotes Macrophage Chemotaxis.; Authors - Nan Li
human	IHC	Front Cell Dev Biol; Integrative Analysis of DNA Methylation and Transcriptome Identifies a Predictive Epigenetic Signature Associated With Immune Infiltration in Gliomas.; Authors - Jianlei Zhang
human	IHC	Front Oncol; Knockdown of SPON2 inhibits the growth of triple-negative breast cancer; Authors - Xueyi Hu