SPON2 Monoclonal antibody

SPON2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1H4B11

Cat no : 66859-1-Ig

Synonyms

DIL 1, DIL1, M spondin, Mindin, SPON2, Spondin 2

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Validation Data Gallery

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  WB analysis of LNCaP using 66859-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66859-1-Ig (SPON2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66859-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66859-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: LNCaP cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

66859-1-Ig targets SPON2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SPON2 fusion protein Ag14337
• Full Name: spondin 2, extracellular matrix protein
• Calculated Molecular Weight: 331 aa, 36 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC002707
• Gene Symbol: SPON2
• Gene ID (NCBI): 10417
• RRID: AB_2882198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).

Publications

Species	Application	Title
human	WB,IHC	Bone; SPON2 promotes the bone metastasis of lung adenocarcinoma via activation of the NF-κB signaling pathway; Authors - Ming Wu ; KD Validated