SPRY1 Polyclonal antibody

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  WB analysis using 17813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17813-1-AP (SPRY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17813-1-AP (SPRY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-29 using 17813-1-AP

  IP result of anti-SPRY1 (IP:17813-1-AP, 4ug; Detection:17813-1-AP 1:3000) with HT-29 cells lysate 1760 ug.

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  IP experiment of A2780 using 17813-1-AP

  IP result of anti-SPRY1 (IP:17813-1-AP, 4ug; Detection:17813-1-AP 1:3000) with A2780 cells lysate 1000 ug.

SPRY1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 17813-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

hSPRY1, Protein sprouty homolog 1, Spry 1, Spry-1

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Tested Applications

• Positive WB detected in: A2780 cells, HEK-293 cells, HepG2 cells
• Positive IP detected in: HT-29 cells, A2780 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17813-1-AP targets SPRY1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12129

  Product name: Recombinant human SPRY1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-319 aa of BC063856

  Sequence: MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPILSRSTSTGSAASSGSNSSASSEQGLLGRSPPTRPVPGHRSERAIRTQPKQLIVDDLKGSLKEDLTQHKFICEQCGKCKCGECTAPRTLPSCLACNRQCLCSAESMVEYGTCMCLVKGIFYHCSNDDEGDSYSDNPCSCSQSHCCSRYLCMGAMSLFLPCLLCYPPAKGCLKLCRRCYDWIHRPGCRCKNSNTVYCKLESCPSRGQGKPS

• Full Name: sprouty homolog 1, antagonist of FGF signaling (Drosophila)
• Calculated Molecular Weight: 319 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC063856
• Gene Symbol: SPRY1
• Gene ID (NCBI): 10252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43609
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sprouty homolog 1 (SPRY1) belongs to the sprouty family. Spry-1 is a negative regulator of receptor tyrosine kinase signalling (PMID: 36543535). Spry1 and its related proteins have also been implicated as negative regulators of RhoA and its downstream effector Rho kinase through the non-canonical Wnt signaling (PMID: 19671746, PMID: 15184877). Sprouty-1 and Sprouty-2 proteins are expressed in early embryonic lung both in the epithelial cells of distal tubules and in peripheral mesenchymal cells (PMID: 22236546). Spry1 is a cytoplasmic protein that plays a critical role in kidney development and is primarily known to inhibit the Ras/MEK/ERK pathway (PMID: 21310958).