Tested Applications
| Positive WB detected in | HepG2 cells, T-47D cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13128-1-AP targets FDFT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3743 Product name: Recombinant human SQS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-358 aa of BC029641 Sequence: MEFVKCLGHPEEFYNLVRFRIGGKRKVMPKMDQDSLSSSLKTCYKYLNQTSRSFAAVIQALDGEMRNAVCIFYLVLRALDTLEDDMTISVEKKVPLLHNFHSFLYQPDWRFMESKEKDRQVLEDFPTISLEFRNLAEKYQTVIADICRRMGIGMAEFLDKHVTSEQEWDKYCHYVAGLVGIGLSRLFSASEFEDPLVGEDTERANSMGLFLQKTNIIRDYLEDQQGGREFWPQEVWSRYVKKLGDFAKPENIDLAVQCLNELITNALHHIPDVITYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGAVKIRKGQAVTLMMDATNMPAVKAIIYQYMEEIYHRIPDSDPSSSK Predict reactive species |
| Full Name | farnesyl-diphosphate farnesyltransferase 1 |
| Calculated Molecular Weight | 417 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC029641 |
| Gene Symbol | FDFT1 |
| Gene ID (NCBI) | 2222 |
| RRID | AB_2294094 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37268 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Farnesyl-diphosphate farnesyltransferase 1 (FDFT1), also known as SQS, is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FDFT1 antibody 13128-1-AP | Download protocol |
| IHC protocol for FDFT1 antibody 13128-1-AP | Download protocol |
| IP protocol for FDFT1 antibody 13128-1-AP | Download protocol |
| WB protocol for FDFT1 antibody 13128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FDFT1 Polyclonal antibody (Cat# 13128-1-AP) has 34 citations. These appear in journals including Nature, Cell Metabolism, Nature Cancer, Nature Communications, Neuron, Molecular Cell, Cancer Research, Oncogene, Cell Death & Disease, Advanced Science, Signal Transduction and Targeted Therapy, Phytomedicine, Journal of Biological Chemistry, iScience, and PeerJ. Research fields center on cholesterol and mevalonate biosynthesis, lipid metabolism, cancer (lung, breast, ovarian, pancreatic, colorectal), ferroptosis, and cardiomyopathy.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy. | ||
Nature Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death. | ||
Cell Metab Functional Genomics Identifies Metabolic Vulnerabilities in Pancreatic Cancer.
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Nat Cancer Therapeutic targeting of the mevalonate-geranylgeranyl diphosphate pathway with statins overcomes chemotherapy resistance in small cell lung cancer. | ||
Cancer Res Caffeine supplementation and FOXM1 inhibition enhance the antitumor effect of statins in neuroblastoma
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