Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, HeLa cells, HepG2 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18699-1-AP targets SRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | steroid receptor RNA activator 1 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 33-35 kDa |
| GenBank Accession Number | BC156760 |
| Gene Symbol | SRA1 |
| Gene ID (NCBI) | 10011 |
| RRID | AB_2270887 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HD15 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SRA1 antibody 18699-1-AP | Download protocol |
| WB protocol for SRA1 antibody 18699-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SRA1 Polyclonal antibody (Cat# 18699-1-AP) has 2 citations. They appear in Translational Neurodegeneration (2024), studying microglial targeting and neuroinflammation in Alzheimer's pathology, and Atherosclerosis (2023), investigating flagellar hook protein FlgE promoting macrophage activation and atherosclerosis via ATP5B targeting. Both studies used mouse models, with applications including WB and IHC. The research fields span neurodegeneration/neuroinflammation and cardiovascular disease/atherosclerosis.
| Species | Application | Title |
|---|---|---|
Transl Neurodegener Nanotechnology for microglial targeting and inhibition of neuroinflammation underlying Alzheimer's pathology | ||
Atherosclerosis Flagellar hook protein FlgE promotes macrophage activation and atherosclerosis by targeting ATP5B |
