SRD5A1 Polyclonal antibody

SRD5A1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26001-1-AP

Synonyms

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

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Validation Data Gallery

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  WB analysis of mouse testis using 26001-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26001-1-AP (SRD5A1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of DU 145 using 26001-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 26001-1-AP (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26001-1-AP (SRD5A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26001-1-AP (SRD5A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, DU 145 cells
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

The immunogen of 26001-1-AP is SRD5A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRD5A1 fusion protein Ag23351
• Full Name: steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
• Observed molecular weight: 29 kDa
• GenBank accession number: BC007033
• Gene symbol: SRD5A1
• Gene ID (NCBI): 6715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Steroid 5-alpha-reductase (SRD5A1) catalyzes the conversion of 17β-Hydroxyandrost-4-en-3-one into the more potent androgen, DHT. SRD5A1 is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.

Publications

Species	Application	Title
rat	WB	Sci Rep; Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5α-Reductase.; Authors - Jiakuan Liu
mouse	WB	J Steroid Biochem Mol Biol; Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells.; Authors - Qi Wang
rat	WB	J Integr Med; Rauwolfia vomitoria extract suppresses benign prostatic hyperplasia by reducing expression of androgen receptor and 5α-reductase in a rat model.; Authors - Tian Fang
mouse	IF	Biomedicines; Mixed-Culture Propagation of Uterine-Tissue-Resident Macrophages and Their Expression Properties of Steroidogenic Molecules; Authors - Kazushige Ogawa