SRP14 Polyclonal antibody

• Featured Product
• KD/KO Validated

SRP14 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 11528-1-AP

Synonyms

18 kDa Alu RNA binding protein, ALURBP, SRP14

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Validation Data Gallery

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  WB analysis using 11528-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11528-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11528-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11528-1-AP

  IP result of anti-SRP14 (IP:11528-1-AP, 4ug; Detection:11528-1-AP 1:300) with A549 cells lysate 1600ug.

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  IF Staining of MCF-7 using 11528-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SRP14 antibody 11528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 11528-1-AP

  Immunofluorescent analysis of HepG2 cells, using SRP14 antibody 11528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, human heart tissue
• Positive IP detected in: A549 cells
• Positive IF detected in: MCF-7 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IP: See 1 publications below

Product Information

11528-1-AP targets SRP14 in WB, IP, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRP14 fusion protein Ag2099
• Full Name: signal recognition particle 14kDa (homologous Alu RNA binding protein)
• Calculated Molecular Weight: 136 aa, 15 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC035495
• Gene Symbol: SRP14
• Gene ID (NCBI): 6727
• RRID: AB_2194708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal recognition particle(SRP) acts in three distinct ways: 1 it binds to the signal sequence of the nascent polypeptide to be translocated, which is exposed on the surface of the translating ribosome; 2 it temporarily retards the nascent polypeptide from further elongation; 3 it mediates docking of the SRP-ribosome-nascent polypeptide chain complex to the RER membrane via the heterodimeric SRP-receptor (SR). SRP14, also named as 18 kDa Alu RNA-binding protein, is a 136 amino acid protein. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. SRP14 protein is a component of signal-recognition-particle that has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. SRP14 form a heterodimer with SRP9, which recognizes Alu RNA and the related 7SL RNA.

Publications

Species	Application	Title
human	WB	Cell; Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.; Authors - Barzin Y Nabet ; KD Validated
human	WB	Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	Genes Dev; Human intron-encoded Alu RNAs are processed and packaged into Wdr79-associated nucleoplasmic box H/ACA RNPs.; Authors - Jády Beáta E BE
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
human	WB	Nucleic Acids Res; Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.; Authors - Piazzon Nathalie N
human	WB	J Biol Chem; The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.; Authors - Evan P Booy