SRP54 Polyclonal antibody

SRP54 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11729-1-AP

Synonyms

SRP54

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Validation Data Gallery

• 

  WB analysis of human placenta using 11729-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11729-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11729-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11729-1-AP (SRP54 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 11729-1-AP

  IP result of anti-SRP54 (IP:11729-1-AP, 4ug; Detection:11729-1-AP 1:500) with human placenta tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11729-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11729-1-AP (SRP54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, NIH/3T3 cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human breast cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

11729-1-AP targets SRP54 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRP54 fusion protein Ag2327
• Full Name: signal recognition particle 54kDa
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC003389
• Gene Symbol: SRP54
• Gene ID (NCBI): 6729
• RRID: AB_2194724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

Publications

Species	Application	Title
human		Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	PLoS One; Decreased dicer expression enhances SRP-mediated protein targeting.; Authors - Ren Yong-Feng YF
human	WB	Biochem Biophys Res Commun; Polyglutamine expansion disturbs the endoplasmic reticulum formation, leading to caspase-7 activation through Bax.; Authors - Masashi Ueda
human	WB	Biochem Biophys Res Commun; Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion.; Authors - Masashi Ueda
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi