Featured Product
KD/KO Validated

SRPK2 Polyclonal antibody

SRPK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 25417-1-AP

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Synonyms

SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2, SRPK2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIPIHC

WB analysis of HeLa using 25417-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 25417-1-AP
WB result of SRPK2 antibody (25417-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRPK2 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 25417-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 25417-1-AP
IP result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 25417-1-AP
IP result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 25417-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 25417-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 25417-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 25417-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 25417-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SRPK2 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse testis tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25417-1-AP targets SRPK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17885 Product name: Recombinant human SRPK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 293-513 aa of BC035214 Sequence: LEREAERKIIEENITSAAPSNDQDGEYCPEVKLKTTGLEEAAEAETAKDNGEAEDQEEKEDAEKENIEKDEDDVDQELANIDPTWIESPKTNGHIENGPFSLEQQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLV Predict reactive species
Full Name	SFRS protein kinase 2
Calculated Molecular Weight	688 aa, 78 kDa
Observed Molecular Weight	105-110 kDa
GenBank Accession Number	BC035214
Gene Symbol	SRPK2
Gene ID (NCBI)	6733
RRID	AB_2880068
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P78362
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).

Protocols

Product Specific Protocols
IHC protocol for SRPK2 antibody 25417-1-AP	Download protocol
IP protocol for SRPK2 antibody 25417-1-AP	Download protocol
WB protocol for SRPK2 antibody 25417-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The SRPK2 Polyclonal antibody (Cat# 25417-1-AP) has 1 citation. It appears in Cancer Discovery (IF 29.5), in a 2022 study by Fonteneau et al. examining how stress granules drive obesity-associated pancreatic cancer development. The research field is oncology, specifically pancreatic cancer biology and stress granule function. The antibody was used for WB and IF applications in human samples.

Species	Application	Title
human	WB,IF	Cancer Discov Stress Granules determine the Development of Obesity-associated Pancreatic Cancer. Authors - Guillaume Fonteneau View Article