SRPK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SRPK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25417-1-AP

Synonyms

SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2, SRPK2

---

Validation Data Gallery

• 

  WB analysis of HeLa using 25417-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25417-1-AP

  WB result of SRPK2 antibody (25417-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRPK2 transfected HeLa cells.

• 

  WB analysis of HeLa using 25417-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25417-1-AP (SRPK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25417-1-AP

  IP result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.

• 

  IP experiment of HeLa using 25417-1-AP

  IP result of anti-SRPK2 (IP:25417-1-AP, 4ug; Detection:25417-1-AP 1:300) with HeLa cells lysate 3200ug.

• 

  IHC staining of mouse testis using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25417-1-AP (SRPK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

25417-1-AP targets SRPK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRPK2 fusion protein Ag17885
• Full Name: SFRS protein kinase 2
• Calculated Molecular Weight: 688 aa, 78 kDa
• Observed Molecular Weight: 105-110 kDa
• GenBank Accession Number: BC035214
• Gene Symbol: SRPK2
• Gene ID (NCBI): 6733
• RRID: AB_2880068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).

Publications

Species	Application	Title
human	WB,IF	Cancer Discov; Stress Granules determine the Development of Obesity-associated Pancreatic Cancer.; Authors - Guillaume Fonteneau