SRPRB Recombinant monoclonal antibody, PBS Only

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• SRPRB Recombinant monoclonal antibody, PBS Only (85926-2-PBS)

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  WB analysis using 85926-2-RR (same clone as 85926-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85926-2-RR (SRPRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85926-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85926-2-RR (same clone as 85926-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85926-2-RR (SRPRB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85926-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85926-2-RR (same clone as 85926-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85926-2-RR (SRPRB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85926-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85926-2-RR (same clone as 85926-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRPRB antibody (85926-2-RR, Clone: 242807E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85926-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85926-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85926-2-RR against Human SRPRB were performed. The affinity constant is 82.7 pM.

SRPRB Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 85926-2-PBS

Clone No.242807E8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein APMCF1, PSEC0230, Signal recognition particle receptor subunit beta, SR-beta

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Product Information

85926-2-PBS targets SRPRB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6215

  Product name: Recombinant human SRPRB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-271 aa of BC065299

  Sequence: MASADSRRVADGGGAGGTFQPYLDTLRQELQQTDPTLLSVVVAVLAVLLTLVFWKLIRSRRSSQRAVLLVGLCDSGKTLLFVRLLTGLYRDTQTSITDSCAVYRVNNNRGNSLTLIDLPGHESLRLQFLERFKSSARAIVFVVDSAAFQREVKDVAEFLYQVLIDSMGLKNTPSFLIACNKQDIAMAKSAKLIQQQLEKELNTLRVTRSAAPSTLDSSSTAPAQLGKKGKEFEFSQLPLKVEFLECSAKGGRGDVGSADIQDLEKWLAKIA

• Full Name: signal recognition particle receptor, B subunit
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC065299
• Gene Symbol: SRPRB
• Gene ID (NCBI): 58477
• RRID: AB_3744478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y5M8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SRPRB is a signal recognition granule receptor β subunit protein, belonging to the GTPase superfamily of transmembrane GTPases. Located on the endoplasmic reticulum membrane, its main function is to anchor the signal recognition granule receptor α subunit to the endoplasmic reticulum membrane, participating in the co-translation of secretory proteins and membrane proteins targeting the endoplasmic reticulum membrane.