SRRM1 Polyclonal antibody, PBS Only

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• SRRM1 Polyclonal antibody, PBS Only (12822-1-PBS)

• 

  WB analysis of mouse liver using 12822-1-AP (same clone as 12822-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12822-1-AP (same clone as 12822-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 12822-1-AP (same clone as 12822-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12822-1-AP (same clone as 12822-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of UV treated HEK-293 using 12822-1-AP (same clone as 12822-1-PBS)

  UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12822-1-AP (same clone as 12822-1-PBS)

  IP result of anti-SRRM1 (IP:12822-1-AP, 4ug; Detection:12822-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SRRM1 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 12822-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

12822-1-PBS targets SRRM1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3513

  Product name: Recombinant human SRRM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC036187

  Sequence: MDAGFFRGTSAEQDNRFSNKQKKLLKQLKFAECLEKKVDMSKVNLEVIKPWITKRVTEILGFEDDVVIEFIFNQLEVKNPDSKMMQINLTGFLNGKNAREFMGELWPLLLSAQENIAGIPSAFLELKKEEIKQRQIEQEKLASMKKQDEDKDKRDKEEKESSREKRERSHSPRRRKSRSPSPRRRSSPVRRERKRSHSRSPRHRTKSRSPSPAPEKKEKTPELPEPSVKVKEPSVQEATSTSDILKVPKPEPIPEPKEPSPEKNSKKEKEKEKTQPRSRSRSKSRSRTRSRSPSHTRPRRRHRSRSRSYSPRRRPSPRRRPSPRRRTPPRRMPPPPRHRRSRSPVRRRRR

• Full Name: serine/arginine repetitive matrix 1
• Calculated Molecular Weight: 904 aa, 102 kDa
• Observed Molecular Weight: 120-160 kDa
• GenBank Accession Number: BC036187
• Gene Symbol: SRRM1
• Gene ID (NCBI): 10250
• RRID: AB_2286613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IYB3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SRRM1, also named as SRM160 and POP101, contains one PWI domain and belongs to the splicing factor SR family. As part of pre- and post-splicing multiprotein mRNP complexes, SRRM1 is involved in numerous pre-mRNA processing events. It promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. SRRM1 stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. It binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. SRRM1 binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. SRRM1, a protein of high molecular weight(120-160kd), has a heavy glycosylation level and is normally highly phosphorylated. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SRRM1.