SRRT Polyclonal antibody, PBS Only

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• SRRT Polyclonal antibody, PBS Only (20353-1-PBS)

• 

  WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 20353-1-AP (same clone as 20353-1-PBS)

  WB result of SRRT antibody (20353-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRRT transfected NIH/3T3 cells. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 20353-1-AP (same clone as 20353-1-PBS)

  IP result of anti-SRRT (IP:20353-1-AP, 4ug; Detection:20353-1-AP 1:30000) with Jurkat cells lysate 1440 ug. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 20353-1-AP (same clone as 20353-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRRT antibody (20353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SRRT Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 20353-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2

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Product Information

20353-1-PBS targets SRRT in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14196

  Product name: Recombinant human SRRT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 527-876 aa of BC000082

  Sequence: LIHTLDDRTQLWASEPGTPPLPTSLPSQNPILKNITDYLIEEVSAEEEELLGSSGGAPPEEPPKEGNPAEINVERDEKLIKVLDKLLLYLRIVHSLDYYNTCEYPNEDEMPNRCGIIHVRGPMPPNRISHGEVLEWQKTFEEKLTPLLSVRESLSEEEAQKMGRKDPEQEVEKFVTSNTQELGKDKWLCPLSGKKFKGPEFVRKHIFNKHAEKIEEVKKEVAFFNNFLTDAKRPALPEIKPAQPPGPAQILPPGLTPGLPYPHQTPQGLMPYGQPRPPILGYGAGAVRPAVPTGGPPYPHAPYGAGRGNYDAFRGQGGYPGKPRNRMVRGDPRAIVEYRDLDAPDDVDFF

• Full Name: serrate RNA effector molecule homolog (Arabidopsis)
• Calculated Molecular Weight: 876 aa, 101 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC000082
• Gene Symbol: SRRT
• Gene ID (NCBI): 51593
• RRID: AB_3085603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXP5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880).