NA14 Polyclonal antibody

NA14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11797-1-AP

Synonyms

N14, NA 14, NA14, Nuclear autoantigen of 14 kDa, SSNA1

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Validation Data Gallery

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  WB analysis of mouse testis using 11797-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11797-1-AP (NA14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11797-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11797-1-AP (NA14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11797-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11797-1-AP (NA14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11797-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11797-1-AP (NA14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 3 publications below

Product Information

11797-1-AP targets NA14 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NA14 fusion protein Ag2367
• Full Name: Sjogren syndrome nuclear autoantigen 1
• Calculated Molecular Weight: 119 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC000864
• Gene Symbol: SSNA1
• Gene ID (NCBI): 8636
• RRID: AB_2195497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SSNA1, also named as NA14 and DIP13, may be an important adaptor to target spastin activity at the centrosome. SSNA1 is a 14 kDa protetin which locates the basal bodies and flagella of human sperm cells and the centrosome of cells.

Publications

Species	Application	Title
	WB	Elife; SSNA1 stabilizes dynamic microtubules and detects microtubule damage.; Authors - Elizabeth J Lawrence
mouse	IF	MBio; The cellular protein La functions in enhancement of virus release through lipid rafts facilitated by murine leukemia virus glycosylated Gag.; Authors - Nitta Takayuki T
mouse	IF	Mol Biol Cell; Functional characterization of putative cilia genes by high-content analysis.; Authors - Lai Cary K CK
human	IF	PLoS One; Spastin-Interacting Protein NA14/SSNA1 Functions in Cytokinesis and Axon Development.; Authors - Uma Goyal
mouse	WB	Biosci Trends; Identification of MIWI-associated Poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody.; Authors - Nishibu Takahiro T