SSSCA1 Polyclonal antibody

• 

  WB analysis of HeLa using 12273-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12273-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12273-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12273-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12273-1-AP

  IP result of anti-SSSCA1 (IP:12273-1-AP, 4ug; Detection:12273-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of mouse testis using 12273-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12273-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 12273-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSSCA1 Polyclonal antibody (12273-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

SSSCA1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 12273-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Synonyms

ZNRD2, Autoantigen p27, p27, Protein ZNRD2, Zinc ribbon domain-containing protein 2

---

Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

12273-1-AP targets SSSCA1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2916

  Product name: Recombinant human SSSCA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-199 aa of BC014791

  Sequence: MALNGAEVDDFSWEPPTEAETKVLQARRERQDRISRLMGDYLLRGYRMLGETCADCGTILLQDKQRKIYCVACQELDSDVDKDNPALNAQAALSQAREHQLASASELPLGSRPAPQPPVPRPEHCEGAAAGLKAAQGPPAPAVPPNTDVMACTQTALLQKLTWASAELGSSTSLETSIQLCGLIRACAEALRSLQQLQH

• Full Name: Sjogren syndrome/scleroderma autoantigen 1
• Calculated Molecular Weight: 199 aa, 21 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC014791
• Gene Symbol: SSSCA1
• Gene ID (NCBI): 10534
• RRID: AB_2195902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60232
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about23- 27 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita ; KO Validated
mouse,human	WB,IHC	J Ethnopharmacol; Centipeda minima extracts and the active sesquiterpene lactones have therapeutic efficacy in non-small cell lung cancer by suppressing Skp2/p27 signaling pathway; Authors - Han-Chen Wang