SSSCA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SSSCA1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12273-1-AP

Synonyms

Autoantigen p27, p27, SSSCA1, ZNRD2

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Validation Data Gallery

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  WB analysis of HeLa using 12273-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12273-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12273-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12273-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12273-1-AP

  IP Result of anti-SSSCA1 (IP:12273-1-AP, 4ug; Detection:12273-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of mouse testis using 12273-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12273-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12273-1-AP

  Immunofluorescent analysis of Hela cells, using SSSCA1 antibody 12273-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 12273-1-AP

  1X10^6 HeLa cells were stained with 0.2 ug SSSCA1 antibody (12273-1-AP) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1500 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1500 (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

12273-1-AP targets SSSCA1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SSSCA1 fusion protein Ag2916
• Full Name: Sjogren syndrome/scleroderma autoantigen 1
• Calculated molecular weight: 199 aa, 21 kDa
• Observed molecular weight: 27 kDa
• GenBank accession number: BC014791
• Gene symbol: SSSCA1
• Gene ID (NCBI): 10534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about23- 27 kDa.

Publications

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita ; KO Validated