Somatostatin Recombinant monoclonal antibody, PBS Only

• 

  IHC staining of mouse pancreas using 84351-1-RR (same clone as 84351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-RR (SST-Specific antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 84351-1-PBS

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-PBS (Somatostatin antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 84351-1-RR (same clone as 84351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-RR (SST-Specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 84351-1-PBS

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-PBS (Somatostatin antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 84351-1-RR (same clone as 84351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Somatostatin antibody (84351-1-RR, Clone: 240816D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84351-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 84351-1-RR (same clone as 84351-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Somatostatin antibody (84351-1-RR, Clone: 240816D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84351-1-PBS in a different storage buffer formulation.

Somatostatin Uni-rAb^® Recombinant Antibody for IHC, IF-P, Indirect ELISA

Cat No. 84351-1-PBS

Clone No.240816D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, Indirect ELISA

Synonyms

SST, somatostatin, SST, SMST, Growth hormone release-inhibiting factor, 240816D9

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Tested Applications

• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20000-1:80000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84351-1-PBS targets Somatostatin in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: somatostatin
• Calculated Molecular Weight: 13 kDa
• GenBank Accession Number: NM_001048
• Gene Symbol: Somatostatin
• Gene ID (NCBI): 6750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P61278
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids.