SSU72 Polyclonal antibody

SSU72 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15434-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

RNA polymerase II subunit A C-terminal domain phosphatase SSU72, PNAS-120, PNAS 120, HSPC182, EC:3.1.3.16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15434-1-AP targets SSU72 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7699

Product name: Recombinant human SSU72 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-194 aa of BC008070

Sequence: SNQNRSMEAHNILSKRGFSVRSFGTGTHVKLPGPAPDKPNVYDFKTTYDQMYNDLLRKDKELYTQNGILHMLDRNKRIKPRPERFQNCKDLFDLILTCEERVYDQVVEDLNSREQETCQPVHVVNVDIQDNHEEATLGAFLICELCQCIQHTEDMENEIDELLQEFEEKSGRTFLHTVCFY

Predict reactive species
Full Name SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae)
Calculated Molecular Weight 23 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC008070
Gene Symbol SSU72
Gene ID (NCBI) 29101
RRIDAB_2878138
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SSU72 antibody 15434-1-APDownload protocol
IHC protocol for SSU72 antibody 15434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency

Authors - Chikako Okubo

Mol Cell

Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors

Authors - Chengjiang Lu
Loading...
||
New chat Able
正在加载,请稍候...