ST3GAL2 Polyclonal antibody

ST3GAL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13620-1-AP

Synonyms

Alpha 2,3 ST 2, Gal NAc6S, Sialyltransferase 4B, SIAT4 B, SIAT4B, ST3Gal II, ST3GAL2, ST3GalA.2, ST3GALII

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Validation Data Gallery

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  WB analysis of human colon using 13620-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13620-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13620-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 13620-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13620-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13620-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human colon tissue, HL-60 cells, human heart tissue, human liver tissue, human spleen tissue, K-562 cells
• Positive IHC detected in: rat heart tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13620-1-AP targets ST3GAL2 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST3GAL2 fusion protein Ag4528
• Full Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 2
• Calculated Molecular Weight: 350 aa, 40 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC036777
• Gene Symbol: ST3GAL2
• Gene ID (NCBI): 6483
• RRID: AB_2286561
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) is a sialyltransferase (ST) that transfers sialic acid from the activated donor CMP-sialic acid to the glycan terminus of a glycolipid or glycoprotein. ST3GAL2 contains two putative N-glycosylation sites (Asn92 and Asn211), which are mainly responsible for GD1a and GT1b ganglioside biosynthesis in the brain. ST3GAL2 belongs to the sialyltransferase family, which mediates the terminal modification of glycoproteins and glycolipids. ST3GAL2 has been found to play a role in obesity, aging, and malignant diseases (PMID: 34319453). The calculated molecular weight of ST3GAL2 is 40 kDa, but the observed is 55 kDa, which may be the glycosylation form of ST3GAL2.

Publications

Species	Application	Title
human,mouse	WB	Int J Biol Macromol; Regulation of autophagy by ST3GAL2-mediated α2-3 sialylated glycosphingolipids in hepatic encephalopathy; Authors - Xiaocheng Li