Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24925-1-AP targets ST3GAL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20795 Product name: Recombinant human ST3GAL3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 203-375 aa of BC050380 Sequence: TTLRITYPEGAMQRPEQYERDSLFVLAGFKWQDFKWLKYIVYKERVSASDGFWKSVATRVPKEPPEIRILNPYFIQEAAFTLIGLPFNNGLMGRGNIPTLGSVAVTMALHGCDEVAVAGFGYDMSTPNAPLHYYETVRMAAIKESWTHNIQREKEFLRKLVKARVITDLSSGI Predict reactive species |
| Full Name | ST3 beta-galactoside alpha-2,3-sialyltransferase 3 |
| Calculated Molecular Weight | 375 aa, 42 kDa |
| Observed Molecular Weight | 44-55 kDa |
| GenBank Accession Number | BC050380 |
| Gene Symbol | ST3GAL3 |
| Gene ID (NCBI) | 6487 |
| RRID | AB_2879799 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q11203 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ST3GAL3 antibody 24925-1-AP | Download protocol |
| WB protocol for ST3GAL3 antibody 24925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ST3GAL3 Polyclonal antibody (catalog number 24925-1-AP) has a total of 1 citation. It appears in FEBS Letters, in a 2018 study titled "The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo," where it was used for WB and IHC in human samples. The associated research field is reproductive biology, specifically uterine endometrial receptivity and sialyltransferase function.
| Species | Application | Title |
|---|---|---|
FEBS Lett The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo.
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