ST3GAL4 Polyclonal antibody

ST3GAL4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13546-1-AP

Synonyms

Alpha 2,3 sialyltransferase IV, Alpha 2,3 ST 4, CGS23, Gal NAc6S, NANTA3, SAT 3, SAT3, Sialyltransferase 4C, SIAT4, SIAT4 C, SIAT4C, ST 4, ST3Gal IV, ST3GAL4, ST3GalA.2, ST3GalIV, STZ



Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13546-1-AP targets ST3GAL4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST3GAL4 fusion protein Ag3665
Full Name ST3 beta-galactoside alpha-2,3-sialyltransferase 4
Calculated Molecular Weight 333 aa, 38 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberBC010645
Gene Symbol ST3GAL4
Gene ID (NCBI) 6484
RRIDAB_10597402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST3GAL4, also known as CGS23, NANTA3 and SIAT4C, is a member of the glycosyltransferase 29 family. ST3GAL4 protein exists in multiple splice variants and is frequently modified by glycosylation. ST3GAL4 is expressed uniformly in tissues and organs during all stages of development, and the subcellular localization is currently not clear.

Protocols

Product Specific Protocols
WB protocol for ST3GAL4 antibody 13546-1-APDownload protocol
IHC protocol for ST3GAL4 antibody 13546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Front Oncol

ST3Gal IV Mediates the Growth and Proliferation of Cervical Cancer Cells In Vitro and In Vivo Via the Notch/p21/CDKs Pathway.

Authors - Yinshuang Wu
humanWB,IHC

Sci Rep

Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.

Authors - Dachang Liu
humanWB,IHC

FEBS Lett

The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo.

Authors - Ming Yu
humanWB

Glycobiology

Sialylation-dependent interaction between PD-L1 and CD169 promotes monocytes adhesion to endothelial cells

Authors - Kebo Cai
mouseIF

Elife

RANK+TLR2+ myeloid subpopulation converts autoimmune to joint destruction in rheumatoid arthritis

Authors - Weixin Zhang