ST3GAL4 Polyclonal antibody

ST3GAL4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13546-1-AP

Synonyms

EC:2.4.3.2, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4, CGS23, Beta-galactoside alpha-2,3-sialyltransferase 4, Alpha 2,3-ST 4

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Validation Data Gallery

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  WB analysis using 13546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13546-1-AP (ST3GAL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13546-1-AP (ST3GAL4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13546-1-AP (ST3GAL4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13546-1-AP (ST3GAL4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 13546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13546-1-AP (ST3GAL4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HCT 116 cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human testis tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

13546-1-AP targets ST3GAL4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST3GAL4 fusion protein Ag3665
• Full Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 4
• Calculated Molecular Weight: 333 aa, 38 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC010645
• Gene Symbol: ST3GAL4
• Gene ID (NCBI): 6484
• RRID: AB_10597402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST3GAL4, also known as CGS23, NANTA3 and SIAT4C, is a member of the glycosyltransferase 29 family. ST3GAL4 protein exists in multiple splice variants and is frequently modified by glycosylation. ST3GAL4 is expressed uniformly in tissues and organs during all stages of development, and the subcellular localization is currently not clear.

Publications

Species	Application	Title
human	WB, IHC	Front Oncol; ST3Gal IV Mediates the Growth and Proliferation of Cervical Cancer Cells In Vitro and In Vivo Via the Notch/p21/CDKs Pathway.; Authors - Yinshuang Wu
human	WB,IHC	Sci Rep; Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.; Authors - Dachang Liu
human	WB,IHC	FEBS Lett; The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo.; Authors - Ming Yu
human	WB	Glycobiology; Sialylation-dependent interaction between PD-L1 and CD169 promotes monocytes adhesion to endothelial cells; Authors - Kebo Cai
mouse	IF	Elife; RANK+TLR2+ myeloid subpopulation converts autoimmune to joint destruction in rheumatoid arthritis; Authors - Weixin Zhang