ST3GAL5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ST3GAL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14614-1-AP

Synonyms

ST3GAL5, ST3GalV, UNQ2510/PRO5998, CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase, EC:2.4.3.9, Lactosylceramide alpha-2,3-sialyltransferase, SIAT9, SIATGM3S, ST3Gal V

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 14614-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14614-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14614-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14614-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14614-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14614-1-AP (ST3GAL5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14614-1-AP (ST3GAL5 Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells, human testis tissue, human placenta tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human skeletal muscle tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

14614-1-AP targets ST3GAL5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST3GAL5 fusion protein Ag6207
• Full Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 5
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC065936
• Gene Symbol: ST3GAL5
• Gene ID (NCBI): 8869
• RRID: AB_2194414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is highly expressed in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). ST3GAL5 has 3 isoforms with the molecular mass of 45, 46, and 48 kDa. Sometimes the band of 70 kDa can also be observed, which may be a glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).

Publications

Species	Application	Title
human	IHC	Front Immunol; Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma; Authors - Jiakuan Liu
human	WB	Front Cell Dev Biol; PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells; Authors - Norihiko Sasaki
human	WB	iScience; C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages; Authors - Lin Tian
human,mouse	WB,IHC,IF	Mol Oncol; Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a pre-metastatic microenvironment; Authors - Misato Horie ; KO Validated
human	WB,IHC	Int Immunopharmacol; Identification and validation of sialyltransferase ST3Gal5 in bladder cancer through bioinformatics and experimental analysis; Authors - Yuli Jian