• Featured Product
  • KD/KO Validated

ST3GAL6 Polyclonal antibody

ST3GAL6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13154-1-AP

Synonyms

Sialyltransferase 10, SIAT10, ST3Gal VI, ST3GAL6, ST3GALVI



Tested Applications

Positive WB detected inMCF-7 cells, human heart tissue, Sp2/0 cells, human placenta tissue
Positive IHC detected inhuman liver tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13154-1-AP targets ST3GAL6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST3GAL6 fusion protein Ag3782
Full Name ST3 beta-galactoside alpha-2,3-sialyltransferase 6
Calculated molecular weight 331 aa, 38 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC023312
Gene symbol ST3GAL6
Gene ID (NCBI) 10402
RRIDAB_10646441
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST3GAL6, also named as SIAT10, belongs to the glycosyltransferase 29 family. ST3GAL6 is involved in the synthesis of sialyl-paragloboside, which is a precursor of sialyl-Lewis X determinant. ST3GAL6 has a alpha-2,3-sialyltransferase activity toward Gal-beta1,4-GlcNAc structure on glycoproteins and glycolipids. ST3GAL6 is known to promote cell adhesion and migration of multiple myeloma cells by increasing the synthesis of selectin ligands. ST3GAL6 has 2 isoforms with the molecular mass of 25 and 38 kDa. Sometimes higher molecular weight about 50-60 kDa can also be observed, which may be a glycosylated modified variant of ST3GAL6. (PMID: 31914669, 30220088)

Protocols

Product Specific Protocols
WB protocol for ST3GAL6 antibody 13154-1-APDownload protocol
IHC protocol for ST3GAL6 antibody 13154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Oncol

lncRNA ST3GAL6‑AS1 promotes invasion by inhibiting hnRNPA2B1‑mediated ST3GAL6 expression in multiple myeloma.

Authors - Ying Shen
  • KD Validated
humanWB,IHC

FEBS Lett

The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo.

Authors - Ming Yu