ST3GAL6 Polyclonal antibody

• Featured Product
• KD/KO Validated

ST3GAL6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13154-1-AP

Synonyms

Sialyltransferase 10, SIAT10, ST3Gal VI, ST3GAL6, ST3GALVI

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Validation Data Gallery

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  WB analysis using 13154-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13154-1-AP (ST3GAL6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13154-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 13154-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 13154-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 13154-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13154-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, human heart tissue, Sp2/0 cells, human placenta tissue
• Positive IHC detected in: human liver tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13154-1-AP targets ST3GAL6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST3GAL6 fusion protein Ag3782
• Full Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 6
• Calculated Molecular Weight: 331 aa, 38 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC023312
• Gene Symbol: ST3GAL6
• Gene ID (NCBI): 10402
• RRID: AB_10646441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST3GAL6, also named as SIAT10, belongs to the glycosyltransferase 29 family. ST3GAL6 is involved in the synthesis of sialyl-paragloboside, which is a precursor of sialyl-Lewis X determinant. ST3GAL6 has a alpha-2,3-sialyltransferase activity toward Gal-beta1,4-GlcNAc structure on glycoproteins and glycolipids. ST3GAL6 is known to promote cell adhesion and migration of multiple myeloma cells by increasing the synthesis of selectin ligands. ST3GAL6 has 2 isoforms with the molecular mass of 25 and 38 kDa. Sometimes higher molecular weight about 50-60 kDa can also be observed, which may be a glycosylated modified variant of ST3GAL6. (PMID: 31914669, 30220088)

Publications

Species	Application	Title
human	WB	Int J Oncol; lncRNA ST3GAL6‑AS1 promotes invasion by inhibiting hnRNPA2B1‑mediated ST3GAL6 expression in multiple myeloma.; Authors - Ying Shen ; KD Validated
human	WB,IHC	FEBS Lett; The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo.; Authors - Ming Yu