ST5 Polyclonal antibody

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  WB analysis of human testis using 12893-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 12893-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12893-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 12893-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 12893-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12893-1-AP (ST5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ST5 Polyclonal Antibody for WB, ELISA

Cat No. 12893-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DENN domain-containing protein 2B, DENND2B, HTS1

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Tested Applications

• Positive WB detected in: human testis tissue, SH-SY5Y cells, HeLa cells, human adrenal gland tissue, mouse uterus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12893-1-AP targets ST5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3403

  Product name: Recombinant human ST5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 792-1137 aa of BC036655

  Sequence: LPEVYCVISRLGCFGLFSKVLDEVERRRGISAALVYPFMRSLMESPFPAPGKTIKVKTFLPGAGNEVLELRRPMDSRLEHVDFECLFTCLSVRQLIRIFASLLLERRVIFVADKLSTLSSCSHAVVALLYPFSWQHTFIPVLPASMIDIVCCPTPFLVGLLSSSLPKLKELPVEEALMVNLGSDRFIRQMDDEDTLLPRKLQAALEQALERKNELISQDSDSDSDDECNTLNGLVSEVFIRFFVETVGHYSLFLTQSEKGERAFQREAFRKSVASKSIRRFLEVFMESQMFAGFIQDRELRKCRAKGLFEQRVEQYLEELPDTEQSGMNKFLRGLGNKMKFLHKKN

• Full Name: suppression of tumorigenicity 5
• Calculated Molecular Weight: 1137 aa, 126 kDa
• Observed Molecular Weight: 82 kDa, 70 kDa
• GenBank Accession Number: BC036655
• Gene Symbol: ST5
• Gene ID (NCBI): 6764
• RRID: AB_10640670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78524
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level).