Tested Applications
| Positive WB detected in | HL-60 cells, RAW 264.7 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28367-1-AP targets ST6GAL2 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26357 Product name: Recombinant human ST6GAL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-200 aa of BC008680 Sequence: SNPAEPVPSSLSFLETRRLLPVQGKQRAIMGAAHEPSPPGGLDARQALPRAHPAGSFHAGPGDLQKWAQSQDGFEHKEFFSSQVGRKSQSAFYPEDDDYFFAAGQPGWHSHTQGTLGFPSPGEPGPREGAFPAAQVQRRRVKKRHRRQRRSHVLEEGDDGDRLYSSMSRAFLYRLWKGN Predict reactive species |
| Full Name | ST6 beta-galactosamide alpha-2,6-sialyltranferase 2 |
| Calculated Molecular Weight | 529 aa, 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC008680 |
| Gene Symbol | ST6GAL2 |
| Gene ID (NCBI) | 84620 |
| RRID | AB_2881124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96JF0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ST6GAL2 antibody 28367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ST6GAL2 Polyclonal antibody (Cat# 28367-1-AP) has a total of 2 citations. These appear in International Immunopharmacology and Journal of Inflammation Research. The associated research fields include osteoarthritis (OA) pathological progression modeling and hepatocellular carcinoma, specifically liver inflammation and adverse prognosis prediction. Both citations used the antibody for Western blot (WB) applications in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Intra-articular injection of Erastin induces OA pathological progression as an experimental model. | ||
J Inflamm Res Downregulation of ST6GAL2 Correlates to Liver Inflammation and Predicts Adverse Prognosis in Hepatocellular Carcinoma |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used the antibody for Western Blot at a 1:1000 dilution and reported that it detected the correct band size in both A549 and HT-29 cell lines, indicating reliable specificity and performance for these cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Faezeh (Verified Customer) (01-29-2023) | It shows a right band size in A549 and HT-29 cell lines.
 |
