ST6GALNAC1 Polyclonal antibody

ST6GALNAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15363-1-AP

Synonyms

HSY11339, Sialyltransferase 7A, SIAT7 A, SIAT7A, ST6GalNAc I, ST6GALNAC1, ST6GalNAcI, STYI

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Validation Data Gallery

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  WB analysis using 15363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15363-1-AP (ST6GALNAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 15363-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 15363-1-AP (ST6GALNAC1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 15363-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15363-1-AP (ST6GALNAC1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 15363-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15363-1-AP (ST6GALNAC1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: LNCaP cells, human stomach tissue, PC-3 cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15363-1-AP targets ST6GALNAC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST6GALNAC1 fusion protein Ag8062
• Full Name: ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 66-69 kDa
• GenBank Accession Number: BC022462
• Gene Symbol: ST6GALNAC1
• Gene ID (NCBI): 55808
• RRID: AB_2286578
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Mol Ther Methods Clin Dev; An etanercept O-glycovariant with enhanced potency.; Authors - Thomas G Biel
		Oncotarget; The androgen receptor controls expression of the cancer-associated sTn antigen and cell adhesion through induction of ST6GalNAc1 in prostate cancer.; Authors - Jennifer Munkley