ST6GALNAC1 Polyclonal antibody

ST6GALNAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15363-1-AP

Synonyms

SIAT7 A, HSY11339, hST6GalNAc-I, GalNAc alpha-2,6-sialyltransferase I, EC:2.4.3.3

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Validation Data Gallery

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  WB analysis using 15363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15363-1-AP (ST6GALNAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 15363-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15363-1-AP (ST6GALNAC1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 15363-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15363-1-AP (ST6GALNAC1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse small intestine tissue, rat colon tissue, rat small intestine tissue
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

15363-1-AP targets ST6GALNAC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST6GALNAC1 fusion protein Ag8062
• Full Name: ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 1
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 66-69 kDa
• GenBank Accession Number: BC022462
• Gene Symbol: ST6GALNAC1
• Gene ID (NCBI): 55808
• RRID: AB_2286578
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST6GALNAC1 encodes a sialyltransferase that acts as a catalyst for the synthesis of cancer-related sialylTn (sTn) antigen critical for cell mobility.ST6GALNAC1 was overexpressed in gastric, breast and prostate cancer cell lines, inducing sTn expression.

Publications

Species	Application	Title
	WB	Mol Ther Methods Clin Dev; An etanercept O-glycovariant with enhanced potency.; Authors - Thomas G Biel
human	IHC	PLoS One; Differential expression of ST6GALNAC1 and ST6GALNAC2 and their clinical relevance to colorectal cancer progression; Authors - Mohammed Saqif Ahmad
		Oncotarget; The androgen receptor controls expression of the cancer-associated sTn antigen and cell adhesion through induction of ST6GalNAc1 in prostate cancer.; Authors - Jennifer Munkley
human	WB	J Adv Res; ST6GALNAC1-mediated sialylation in uterine endometrial epithelium facilitates the epithelium-embryo attachment; Authors - Xinyue Dong