Filter: AllWBIHCIF-P

WB analysis of COLO 320 using 66209-1-Ig (same clone as 66209-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 66209-1-Ig (same clone as 66209-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.
IHC staining of human skin using 66209-1-Ig (same clone as 66209-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 66209-1-Ig (same clone as 66209-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ST6GALNAC6 antibody (66209-1-Ig, Clone: 1A7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

ST6GALNAC6 Monoclonal antibody, PBS Only

ST6GALNAC6 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66209-1-PBS

Clone No.1A7B7

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6, EC:2.4.99.-, GalNAc alpha-2,6-sialyltransferase VI, hST6GalNAc VI, RP11 203J24.3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66209-1-PBS targets ST6GALNAC6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8358 Product name: Recombinant human ST6GALNAC6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 67-333 aa of BC007802 Sequence: VFHYGSLRGRSRRPVNLKKWSITDGYVPILGNKTLPSRCHQCVIVSSSSHLLGTKLGPEIERAECTIRMNDAPTTGYSADVGNKTTYRVVAHSSVFRVLRRPQEFVNRTPETVFIFWGPPSKMQKPQGSLVRVIQRAGLVFPNMEAYAVSPGRMRQFDDLFRGETGKDREKSHSWLSTGWFTMVIAVELCDHVHVYGMVPPNYCSQRPRLQRMPYHYYEPKGPDECVTYIQNEHSRKGNHHRFITEKRVFSSWAQLYGITFSHPSWT Predict reactive species
Full Name	ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
Calculated Molecular Weight	333aa,38 kDa; 374aa,41 kDa
Observed Molecular Weight	38-42 kDa
GenBank Accession Number	BC007802
Gene Symbol	ST6GALNAC6
Gene ID (NCBI)	30815
RRID	AB_2881600
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q969X2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.