ST8SIA2-Specific Polyclonal antibody

ST8SIA2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19736-1-AP

Synonyms

Alpha 2,8 sialyltransferase 8B, HsT19690, Sialyltransferase 8B, Sialyltransferase X, Sialytransferase St8Sia II, SIAT8 B, SIAT8B, ST8SIA II, ST8SIA2, ST8SIA2-Specific, ST8SiaII, STX

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Validation Data Gallery

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  WB analysis of mouse heart using 19736-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19736-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 19736-1-AP (ST8SIA2-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 19736-1-AP (ST8SIA2-Specific Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

19736-1-AP targets ST8SIA2-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_006011
• Gene Symbol: ST8SIA2
• Gene ID (NCBI): 8128
• RRID: AB_10638471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST8SIA2, also named as SIAT8B and STX, belongs to the glycosyltransferase 29 family. ST8SIA2 may transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression. The antibody is specific to ST8SIA2.

Publications

Species	Application	Title
mouse,rat	WB,IHC	Oxid Med Cell Longev; Long Noncoding RNA TUG1 Aggravates Cerebral Ischemia/Reperfusion Injury by Acting as a ceRNA for miR-3072-3p to Target St8sia2.; Authors - Miao Chen
human	WB,IF	J Cell Sci; Prolyl endopeptidase is involved in the degradation of neural cell adhesion molecules in vitro.; Authors - Külli Jaako
mouse	WB	Brain Res; PSA modification of NCAM supports the survival of injured retinal ganglion cells in adulthood.; Authors - Natalia Lobanovskaya
human	IHC	Front Immunol; A novel ganglioside-related risk signature can reveal the distinct immune landscape of neuroblastoma and predict the immunotherapeutic response; Authors - Jiaxing Yang