STAC3 Polyclonal antibody

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  WB analysis using 20392-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20392-1-AP (STAC3 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 20392-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 20392-1-AP (STAC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 20392-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 20392-1-AP (STAC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 20392-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20392-1-AP (STAC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20392-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20392-1-AP (STAC3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 20392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using STAC3 antibody (20392-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skeletal muscle using 20392-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using STAC3 antibody (20392-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

STAC3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 20392-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

SH3 and cysteine-rich domain-containing protein 3, SH3 and cysteine rich domain 3

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human gliomas tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

20392-1-AP targets STAC3 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14232

  Product name: Recombinant human STAC3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-325 aa of BC008069

  Sequence: MELPPEPQANGEAVGAGGGPIYYIYEEEEEEEEEEEEPPPEPPKLVNDKPHKFKDHFFKKPKFCDVCARMIVLNNKFGLRCKNCKTNIHEHCQSYVEMQRCFGKIPPGFHRAYSSPLYSNQQYACVKDLSAANRNDPVFETLRTGVIMANKERKKGQADKKNPVAAMMEEEPESARPEEGKPQDGNPEGDKKAEKKTPDDKHKQPGFQQSHYFVALYRFKALEKDDLDFPPGEKITVIDDSNEEWWRGKIGEKVGFFPPNFIIRVRAGERVHRVTRSFVGNREIGQITLKKDQIVVQKGDEAGGYVKVYTGRKVGLFPTDFLEEI

• Full Name: SH3 and cysteine rich domain 3
• Calculated Molecular Weight: 364 aa, 42 kDa
• Observed Molecular Weight: 42-55 kDa
• GenBank Accession Number: BC008069
• Gene Symbol: STAC3
• Gene ID (NCBI): 246329
• RRID: AB_10693618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96MF2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CoIP	Hum Mutat; STAC3 variants cause a congenital myopathy with distinctive dysmorphic features and malignant hyperthermia susceptibility.; Authors - Irina Zaharieva
mouse	WB	Structure; Multiple Sequence Variants in STAC3 Affect Interactions with CaV1.1 and Excitation-Contraction Coupling.; Authors - Britany Rufenach ; KO Validated
mouse	WB	Skelet Muscle; The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle.; Authors - Xiaofei Cong ; KO Validated
human	IF	J Cell Physiol; STAC3 incorporation into skeletal muscle triads occurs independent of the dihydropyridine receptor.; Authors - Marta Campiglio
human	WB	J Gen Physiol; Calcium current modulation by the γ1 subunit depends on alternative splicing of CaV1.1.; Authors - Yousra El Ghaleb
rat	WB	J Appl Physiol (1985); Preconditioning contractions prevent prolonged force depression and Ca2+-dependent proteolysis of STAC3 after damaging eccentric contractions.; Authors - Yuki Ashida