Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human gliomas tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20392-1-AP targets STAC3 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14232 Product name: Recombinant human STAC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC008069 Sequence: MELPPEPQANGEAVGAGGGPIYYIYEEEEEEEEEEEEPPPEPPKLVNDKPHKFKDHFFKKPKFCDVCARMIVLNNKFGLRCKNCKTNIHEHCQSYVEMQRCFGKIPPGFHRAYSSPLYSNQQYACVKDLSAANRNDPVFETLRTGVIMANKERKKGQADKKNPVAAMMEEEPESARPEEGKPQDGNPEGDKKAEKKTPDDKHKQPGFQQSHYFVALYRFKALEKDDLDFPPGEKITVIDDSNEEWWRGKIGEKVGFFPPNFIIRVRAGERVHRVTRSFVGNREIGQITLKKDQIVVQKGDEAGGYVKVYTGRKVGLFPTDFLEEI Predict reactive species |
| Full Name | SH3 and cysteine rich domain 3 |
| Calculated Molecular Weight | 364 aa, 42 kDa |
| Observed Molecular Weight | 42-55 kDa |
| GenBank Accession Number | BC008069 |
| Gene Symbol | STAC3 |
| Gene ID (NCBI) | 246329 |
| RRID | AB_10693618 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96MF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STAC3 antibody 20392-1-AP | Download protocol |
| IHC protocol for STAC3 antibody 20392-1-AP | Download protocol |
| WB protocol for STAC3 antibody 20392-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STAC3 antibody (Cat# 20392-1-AP) has 12 total citations. These appear in journals including JCI Insight, Am J Physiol Cell Physiol, Structure, J Cell Physiol, Skelet Muscle, J Appl Physiol, J Gen Physiol, Am J Cancer Res, Physiol Rep, and Hum Mutat. Research fields span skeletal muscle excitation-contraction coupling, calcium signaling, muscle pathology and myopathy, and cancer biomarker studies. Applications include WB, IF, IHC, and CoIP across mouse, rat, and human species.
| Species | Application | Title |
|---|---|---|
JCI Insight STAC3 binding to CaV1.1 II-III loop is nonessential but critically supports skeletal muscle excitation-contraction coupling.
| ||
Am J Physiol Cell Physiol Dissociation of SH3 and cysteine-rich domain 3 and junctophilin 1 from dihydropyridine receptor in dystrophin-deficient muscles | ||
Am J Physiol Cell Physiol Intrinsic contractile dysfunction due to impaired sarcoplasmic reticulum Ca2+ release in compensatory hypertrophied muscle fibers following synergist ablation | ||
Structure Multiple Sequence Variants in STAC3 Affect Interactions with CaV1.1 and Excitation-Contraction Coupling.
| ||
J Cell Physiol STAC3 incorporation into skeletal muscle triads occurs independent of the dihydropyridine receptor. | ||
Skelet Muscle The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle.
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