STAG3 Polyclonal antibody

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• STAG3 Polyclonal antibody (23314-1-AP)

STAG3 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Cat No. 23314-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, ELISA

Synonyms

Cohesin subunit SA 3, SCC3 homolog 3, STAG3, stromal antigen 3, Stromalin 3

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  IP experiment of human testis using 23314-1-AP

  IP result of anti-STAG3 (IP:23314-1-AP, 4ug; Detection:23314-1-AP 1:300) with human testis tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human testis using 23314-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human testis using 23314-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 23314-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 23314-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 23314-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 23314-1-AP (STAG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 23314-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 23314-1-AP (STAG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IP detected in: human testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells, MCF-7 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23314-1-AP targets STAG3 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19818

  Product name: Recombinant human STAG3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 926-1225 aa of BC146806

  Sequence: RILLLSLKQLYTELLQEHGPQGLNELPAFIEMRDLARRFALSFGPQQLQNRDLVVMLHKEGIQFSLSELPPAGSSNQPPNLAFLELLSEFSPRLFHQDKQLLLSYLEKCLQHVSQAPGHPWGPVTTYCHSLSPVENTAETSPQVLPSSKRRRVEGPAKPNREDVSSSQEESLQLNSIPPTPTLTSTAVKSRQPLWGLKEMEEEDGSELDFAQGQPVAGTERSRFLGPQYFQTPHNPSGPGLGNQLMRLSLMEEDEEEELEIQDESNEERQDTDMQASSYSSTSERGLDLLDSTELDIEDF

• Full Name: stromal antigen 3
• Calculated Molecular Weight: 1225 aa, 139 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC146806
• Gene Symbol: STAG3
• Gene ID (NCBI): 10734
• RRID: AB_2879253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJ98
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The STAG3 Polyclonal antibody (Cat# 23314-1-AP) has 2 total citations. One appeared in Frontiers in Oncology, studying ectopic expression of meiosis regulatory genes in cutaneous T-cell lymphomas using IHC. The other was published in Frontiers in Genetics, investigating in-frame variants in the STAG3 gene causing premature ovarian insufficiency, using IF. Both citations involve human samples and focus on reproductive biology and oncology research fields.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Oncol; The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL).; Authors - Jennifer Gantchev
human	IF	Front Genet; In-Frame Variants in STAG3 Gene Cause Premature Ovarian Insufficiency.; Authors - Wen-Juan Xiao