Tested Applications
| Positive WB detected in | COLO 320 cells, HEK-293T cells, HepG2 cells, THP-1 cells |
| Positive IHC detected in | mouse liver tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10487-1-AP targets STARD5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0753 Product name: Recombinant human STARD5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-213 aa of BC004365 Sequence: MDPALAAQMSEAVAEKMLQYRRDTAGWKICREGNGVSVSWRPSVEFPGNLYRGEGIVYGTLEEVWDCVKPAVGGLRVKWDENVTGFEIIQSITDTLCVSRTSTPSAAMKLISPRDFVDLVLVKRYEDGTISSNATHVEHPLCPPKPGFVRGFNHPCGCFCEPLPGEPTKTNLVTFFHTDLSGYLPQNVVDSFFPRSMTRFYANLQKAVKQFHE Predict reactive species |
| Full Name | StAR-related lipid transfer (START) domain containing 5 |
| Calculated Molecular Weight | 23.4 kDa |
| Observed Molecular Weight | 24-26 kDa |
| GenBank Accession Number | BC004365 |
| Gene Symbol | STARD5 |
| Gene ID (NCBI) | 80765 |
| RRID | AB_2197808 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NSY2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STARD5 (StAR-related lipid transfer domain containing 5) is a cytosolic lipid-transfer protein with a conserved START domain. It binds cholesterol and 25-hydroxycholesterol, maintaining intracellular sterol homeostasis. Its expression is induced under endoplasmic reticulum stress, triggering protein redistribution from nucleus to cytosol and membranes. STARD5 is highly expressed in liver, kidney and spleen; dysregulation links to hepatic steatosis, liver fibrosis and colorectal cancer progression by altering lipid metabolism. (PMID: 41954600)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STARD5 antibody 10487-1-AP | Download protocol |
| IHC protocol for STARD5 antibody 10487-1-AP | Download protocol |
| WB protocol for STARD5 antibody 10487-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The STARD5 Polyclonal antibody (Cat# 10487-1-AP) has 2 citations. One appears in International Journal of Oncology (2024), investigating how AUP1/KDM5B reprograms lipid metabolism in cervical cancer progression. The other is in PLoS One, characterizing dynamic transcriptomic and proteomic profiles of hematopoiesis and hepatogenesis in midtrimester fetal liver. Both studies used the antibody for Western blot in human and mouse samples, respectively.
| Species | Application | Title |
|---|---|---|
Int J Oncol AUP1 transcriptionally activated by KDM5B reprograms lipid metabolism to promote the malignant progression of cervical cancer | ||
PLoS One Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles. |
